Development of a method using immuno-Raman for the simultaneous imaging of 15 biomarkers for immunotherapy of breast cancer.

We propose a novel approach that will provide researchers with an improved method to study multiple molecules, simultaneously in the same tissue section. Our strategy is to use novel nanoparticles that each emit unique multispectral signature and can be used simultaneously. We aim to develop a novel method to detect 15 breast cancer and immunotherapy-related biomarkers at the same time. The capability to detect a multitude of biomarkers simultaneously on the same tissue section would provide unprecedented headways in the field of cancer research and particularly on tumor profiling.