PDF - Characterization and quantification of adsorbed antigens in adjuvanted vaccines - ON-163

Preferred Disciplines: Immunology (Post-Doc)
Company: Sanofi Pasteur
Project Length: 4-6 months (1 unit)
Desired start date: January 2019
Location: Toronto, ON
No. of Positions: 1
Preferences: None

About the Company: 

Sanofi Pasteur is a global company that provides more than one billion doses of vaccine each year, making it possible to immunize more than 500 million people across the globe. A world leader in the vaccine industry, Sanofi Pasteur offers the broadest range of vaccines protecting against 20 infectious diseases. 

Project Description:

The evaluation of adsorbed antigens in the adjuvanted vaccines is crucial in order to assess the stability and integrity of antigens. The efficacy of adsorbed antigens also assist in finding the best vaccine formulations and bioactivity. The traditional methods like pH altering or desorption by various detergents (e.g. sodium citrate, urea, or phosphate) are often not successful in completely recovering adsorbed antigens. In addition, these methods may alter or denature the antigens during desorption. Therefore, it would be beneficial to evaluate the structural and stability of the adsorbed antigens and characterize distribution of each antigens on adjuvants without desorption.

More recently, a flow cytometry based method was developed to directly quantify the amount of two adsorbed Neisseria meningitidis antigens on aluminum hydroxide as adjuvant.  Although this approach was able to quantify the amount of adsorbed antigens but it was not able to detect the morphology of each particle or characterize distribution of the antigens on adjuvants.

We would like to develop accurate, precise and stability-indicating approach to quantify proteins adsorbed on the surface of adjuvant suspensions through new strategies including a new platform called Image Stream Mark II. This platform combines the quantitative power of large sample sizes with the high information content present in microscopic images allowing the user to derive an unprecedented level of biological information on an object-by-object basis. Herein, we hypothesize that the Image Stream Mark II platform is able to determine antigen identity and concentration by direct analysis of adsorbed antigens in our adjuvanted vaccines. We also believe that in contrast with flow cytometry, the Image Stream Mark II is able to evaluate the distribution of the adsorbed antigens on adjuvants or particle delivery systems.

Research Objectives:

To develop accurate, precise and stability-indicating approach to quantify proteins adsorbed on the surface of adjuvant suspensions.

Methodology:

Image Stream Mark II, Flow cytometry, ELISA, MSD, Luminex, etc. 

Expertise and Skills Needed:

    • Immunology
    • Vaccinology 

    For more info or to apply to this applied research position, please

    1. Check your eligibility and find more information about open projects
    2. Interested students need to get the approval from their supervisor and send their CV along with a link to their supervisor’s university webpage by applying through the webform.
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