PDF - Synthesis of modified dNTPs for enzyme DNA synthesis - ON-179

Preferred Disciplines: Biotechnology, Organic Synthesis, Nucleotide Synthesis (Post-Doc)
Company: Spindle Biotech Inc.
Project Length: 4-6 months (1 unit)
Desired start date: As soon as possible
Location: Toronto, ON
No. of Positions: 1
Preferences: Southeastern Ontario Universities (Toronto, Scarborough). This posting is looking for a PDF to work with Dr. Bernie Kraatz at the University of Toronto Scarborough.

About the Company: 

Spindle Biotech is working on the next generation DNA synthesis using enzyme technology. Our product is a Benchtop DNA Synthesizer with consumable synthesis cartridges. The synthesizer will be installed in research laboratories for a rapid turnaround of long synthetic DNA.

Project Description:

We are currently developing 3’-modified deoxyribonucleoside 5'-triphosphates (3’-modified dNTPs) for our enzyme DNA synthesis platform. We need a scientist to help us develop the said modified bases.

Research Objectives:

  • Synthesize 3’-modified dNTPs for enzyme DNA synthesis purposes (For example, please read SI Appendix of “Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection” F. Chen, E. A. Gaucher, et al., 2010)
  • Develop novel 3’ and 4’ protecting groups


  • To be determined

Expertise and Skills Needed:

  • Candidate should have expertise in organic synthesis
  • Candidate should be able to scale up and troubleshoot existing protocols
  • Candidate should have experience working with standard laboratory equipment and specialized instruments such as Preparative HPLC, Flash Chromatography or NMR
  • Candidate should communicate effectively in writing and orally

For more info or to apply to this applied research position, please

  1. Check your eligibility and find more information about open projects
  2. Interested students need to get the approval from their supervisor and send their CV along with a link to their supervisor’s university webpage by applying through the webform