Post-Doc: The effects of long double-stranded (ds) RNA on the innate immune response - ON-226

Preferred Disciplines: Cell biology and innate immunity
Company: Centre for the Commercialization of Antibodies and Biologics
Project Length: 1 year
Desired start date: Flexible, preferrably May-June 2019  
Location: Waterloo, ON
No. of Positions: 1
Preferences: This position is only available in Dr. Stephanie DeWitte-Orr’s research group at Wilfrid Laurier University

About the Company: 

The Centre for the Commercialization of Antibodies and Biologics (CCAB) is a national Centre of Excellence for Commercialization and Research located in Toronto, Canada. CCAB supports innovators and emerging life sciences companies through its expertise in product and business development to advance therapeutic candidates and technologies that will form the basis for company growth and sustainability.

Project Description:

The project on which we are needing a PDF will focus on dsRNA and its ability to modulate the innate immune response at the cellular level. We are particularly interested in dsRNA’s ability to funnel into the RNAi pathway. The project will focus on therapeutically relevant primary human cell types, such as neurons, lung, retina and intervertebral discs, and will study how these cells respond to dsRNA-mediated RNAi pathways. Once cell lines that support dsRNA-mediated RNAi pathways are identified, the ability for dsRNA to limit virus replication will be examined. 

Research Objectives:

  • Test primary, human cell types for their ability to respond to dsRNA by RNAi
  • Test whether these dsRNA molecules are effective antivirals using RNAi

Methodology:

  • dsRNA synthesis
  • Human cell transfections
  • Measuring fluorescent protein production by plate reader or microscopy
  • TCID50/mL or plaque assay for virus quantification

Expertise and Skills Needed:

  • Human cell culture
  • Molecular biology (qRT-PCR)
  • Microscopy (confocal, fluorescence)
  • Virology experience an asset
  • Strong organizational and record keeping skills
  • Ability to work as a team player
  • Effective communication skills

For more info or to apply to this applied research position, please

  1. Check your eligibility and find more information about open projects
  2. Interested students need to get the approval from their supervisor and send their CV along with a link to their supervisor’s university webpage by applying through the webform.
Program: