Development of miniature fluorescence microscope with optogenetically synchronized patterned illumination - Year two

We propose development of miniature fluorescent microscope with flexible spatiotemporal photostimulation patterns of ensembles of neurons in freely behaving animals.For precision and targeted photostimulation of light-activated proteins (opsins), the light beam from high power incoherent light source will be shaped by user-controlled digital micromirror device (DMD). The light patterns formed by DMD will be sent via fiber bundle to miniature imaging system that can be implanted on the head of the animal.

A dual-band optogenetic headstage

B. Gosselin and Doric Lenses Inc. have developed the first low-cost wireless head mounted optogenetic device enabling simultaneous biopotential recording and optical stimulation in the brain of freely moving rodents. This project aims at providing such a wireless optogenetic headstage system with user programmable functions, so it can address the dual nature of brain biopotentials, and become an essential tool for innovative research.