Exploring the potential synergistic cooperation between cellulase enzymes and lignin biocatalysts screened from a proprietary environmental DNA platform for deconstructing lignocellulosic biomass

MetaMixis, Inc., is a Canadain company undertaking a global effort dedicated to decreasing our reliance on petrochemicals by transitioning to engineered microbial processes or biocatalysts for the production of sustainable products. In the past few years MetaMixis has successfully developed a functional screen to recover metagenomic scaffolds sourced from various proprietary bacterial communities.

Exploring the potential synergistic cooperation between cellulase enzymes and lignin biocatalysts screened from a proprietary environmental DNA platform for deconstructing lignocellulosic biomass

MetaMixis, Inc., is a Canadain company undertaking a global effort dedicated to decreasing our reliance on petrochemicals by transitioning to engineered microbial processes or biocatalysts for the production of sustainable products. In the past few years MetaMixis has successfully developed a functional screen to recover metagenomic scaffolds sourced from various proprietary bacterial communities.

High-Throughput Charaterization of Metagenomic Glycosidase Library

Enzymes, Nature’s catalysts, are increasingly used for the degradation of complex sugars such as starch and cellulose in industries such as food processing, brewing and biofuels. New enzymes are normally found by the slow process of assaying individual cultured microorganisms. Discovery can be speeded up enormously through metagenomics, wherein DNA is extracted from samples such as soil and useful genes, hence enzymes, identified by sequencing and screening. Importantly this also allows access to the otherwise inaccessible 95% of genes from non-culturable organisms.

METAGENOMIC SEARCH FOR GLYCOSIDEPHOSPHORYLASES USING A PHOSPHATE DEPENDENT2,4-DINITROPHENYL GLYCOSIDE COLORIMETRICASSAY

Carbohydrate active enzymes are used widely in the commercial sector and in industries ranging from food processing to biofuel generation. Of these enzymes, the class known as glycoside phosphorylases has seemingly been overlooked by industry even given their noted advantages over related classes currently in use. Given the fact that a limited number of these enzymes have been identified, we think this presents a timely niche opportunity to develop a commercially available suite of these enzymes in collaboration with MetaMixis.

Discovery & optimisation of bacterial lignin degradation pathways for the manufacture of value-added chemicals

The design of sustainable manufacturing processes that convert renewable feedstocks into valuable products under clean and ‘green’ operating conditions is essential to ensuring continued economic development and stewardship of the environment. To this end, the use of engineered biological systems to convert biomass into useful chemicals could greatly diminish society’s dependence on petroleum and improve its environmental footprint.

Analytical chemistry method development for lignin breakdown products

The partner organization MetaMixis Inc. is attempting to use biocatalytic ways to process lignin, the second most abundant biopolymer on earth, with microbiology methods to transform lignin into fine chemicals in useful quantities within a reasonable time frame. The process and end products need to be fully characterized using analytical tools to ensure the efficiency. The end products include vanillin, vanillic acid, p-coumaric acid, syringaldehyde, syringic acid, and vanillyl alcohol.