Monitoring biodiversity using DNA-based approaches has many advantages for large-scale studies. DNA metabarcoding can assess the presence, absence, and abundance of species in a sample where individuals are not morphologically identifiable, such as bulk samples of larval fishes or from environmental DNA (eDNA) in water samples. We propose to develop a high-throughput metabarcoding-based approach that will quantify fishes entrained by the water-cooling system at Bruce Power.
All species on the planet are affected by their environment: temperature, atmospheric pressure, daylight hours, and so on. These conditions affect how a species react to stress, grow, reproduce, and survive. Natural background radiation (NBR) is ever-present and has been prior to the evolution of all species. In order to study the effect of NBR on important biological processes, such as brain growth and development, we must remove it from the environment to see if it induces any changes. In order to remove NBR, a deep underground laboratory, shielded from NBR exposure must be used.
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