Cyclin-dependent kinase-2 (CDK2) regulates cell cycle, whose structure and biological characteristics are well known. CRIF1 plays a regulatory role in the bone marrow microenvironment-induced leukemia cell cycle arrest through inhibiting CDK2. In this multi-disciplinary research, we will use a combined structure function study to reveal the interaction between CDK2 and CRIF1. This will facilitate the design of inhibitors for the interaction of the two important proteins, for eventual treatment of leukemia.
Human Crif1 is a protein with multiple functions, playing important roles in embryonic development, cellular stress, cell cycle regulation and mitochondrial membrane integrity. CRIF1 is coined to play a regulatory role in the Bone Marrow microenvironment-induced leukemia cell cycle arrest possibly through interacting with CDK2 and acting as a cyclin-dependent kinase inhibitor.
In this project, we will express human Crif1 protein in baculovirus system, purify it with affinity chromatography and followed by crystallisation.