Somatic cell reprogramming as a tool for the conservation of genetic resources from endangered species

Conservation of endangered species has become increasingly urgent. This is evident given the rate of species extinction by 100 to 1000-fold, and global biodiversity has decreased. With current climate changes, these concerns will only grow. Assisted reproductive technologies can play a vitalrole in endangered species conservation. Many approaches are currently being utilized or explored, including animal cloning. Unfortunately, cloned animals are not genetically pure and therefore, are not valuable for use in breeding programs.

Somatic cell reprogramming as a tool for the conservation of genetic resources from endangered species

Conservation of endangered species has become increasingly urgent. This is evident given the rate of species extinction has increased by 100 to 1000-fold, and global biodiversity has decreased. With current climatic changes, these concerns will only grow. Assisted reproductive technologies can play a vital role in endangered species conservation. Many approaches are currently being utilized or explored, including animal cloning. Unfortunately, cloned animals are not genetically pure and therefore are not valuable for use in breeding programs.

Optimization of wood bison interspecific somatic cell nuclear transfer and embryo culture for species conservation

Interspecies somatic cell nuclear transfer (iSCNT) is a promising technique whereby a bison donor cell is transplanted into a domestic cattle oocyte. Different fibroblast donor cells have been demonstrated to have different embryonic outcomes. Problems with SCNT embryos have been also reported after in vitro culture. Toronto Zoo’s efforts have focused on wood bison iSCNT, a threatened Canadian species, in order to conserve its germplasm.

Influence of nuclear cytoplasmic interactions on developmental competence of bison

Somatic cell nuclear transfer (SCNT) is a unique technology that allows the preservation of the entire genome from an individual, thereby avoiding dilution of valuable alleles. This is an important criterion in endangered species preservation. Our interest in the Canadian wood bison, a threatened species, has brought us to consider the application of SCNT as a method for embryo production and genome preservation. This project will evaluate the developmental competence of bison embryos produced by interspecies SCNT, whereby a bison donor cell is transplanted into a domestic cattle oocyte.

Cellular factors influencing the developmental potential of pure and hybrid bison

Assisted reproductive technologies can provide unique alternatives for wildlife managers interested in preserving genetically valuable individuals. The Canadian wood bison, currently listed as threatened, will benefit greatly from the use of in vitro fertilization and embryo banking as a method for producing genetically healthy, disease‐free herds. However, minimal or non‐existent knowledge of the fundamental aspects of reproductive biology has limited the success of reproductive technologies in non‐domestic species.