Understanding social reproductive suppression across species: zoos as a unique opportunity for research

Social reproductive suppression is when the reproduction of healthy, adult animals in a population is inhibited by the presence of a socially dominant individual. This is known to happen some group-living species, where only the dominant pair will reproduce, and the other group members will assist in raising their young. There is evidence that social reproductive suppression occurs in some non-group living species, but it is unknown how frequently this happens and what evolutionary conditions or species characteristics are associated with its presence.

Understanding the Role of Ex Situ Conservation to Enhance Public Understanding, Awareness, and Support for Biodiversity - Year two

The purpose of this research is to use an evidence-based approach to further the Toronto Zoo’s social science and education programs, in order to support in situ conservation and biodiversity in relation to Canadian goals and the Aichi Targets. Using a collaborative approach this research will result in the co-production of knowledge, where the researcher and the partner organization collaborate in all four stages of the research project. This project is mutually beneficial for the researcher and the partner organization.

Technological advancements for data collection in animal research

The proposed research project is to program artificial intelligence, as produced by technology company EAIGLE, to monitor animals’ behaviour at the Toronto Zoo. The program will be capable of distinguishing where animals are in their enclosures, between individual animals, and which behaviours they are producing under different contexts. This technology will allow zoos, conservation areas, and researchers to monitor how animals interact with their enclosures and throughout the day, allowing for improved habitats and improved data collection for future experiments. Dr.

Production of in vitro and in vivo bison embryos derived from sexed semen - Year two

In mammals, the sperm determines the sex of the resulting offspring. Semen sexing is a process whereby sperm are sorted into Y- (male) or X-chromosome (female) bearing gametes. Sexed semen may be used for artificial insemination or in vitro embryo production to create offspring of a desired sex. In a zoo setting, fewer males are required because of their ability to breed multiple females.

Understanding the Role of Ex Situ Conservation to Enhance Public Understanding, Awareness, and Support for Biodiversity

The purpose of this research is to use an evidence-based approach to further the Toronto Zoo’s social science and education programs, in order to support in situ conservation and biodiversity in relation to Canadian goals and the Aichi Targets. Using a collaborative approach this research will result in the co-production of knowledge, where the researcher and the partner organization collaborate in all four stages of the research project. This project is mutually beneficial for the researcher and the partner organization.

Production of in vitro and in vivo bison embryos derived from sexed semen

In mammals, the sperm determines the sex of the resulting offspring. Semen sexing is a process whereby sperm are sorted into Y- (male) or X-chromosome (female) bearing gametes. Sexed semen may be used for artificial insemination or in vitro embryo production to create offspring of a desired sex. In a zoo setting, fewer males are required because of their ability to breed multiple females.

Somatic cell reprogramming as a tool for the conservation of genetic resources from endangered species

Conservation of endangered species has become increasingly urgent. This is evident given the rate of species extinction by 100 to 1000-fold, and global biodiversity has decreased. With current climate changes, these concerns will only grow. Assisted reproductive technologies can play a vitalrole in endangered species conservation. Many approaches are currently being utilized or explored, including animal cloning. Unfortunately, cloned animals are not genetically pure and therefore, are not valuable for use in breeding programs.

Somatic cell reprogramming as a tool for the conservation of genetic resources from endangered species

Conservation of endangered species has become increasingly urgent. This is evident given the rate of species extinction has increased by 100 to 1000-fold, and global biodiversity has decreased. With current climatic changes, these concerns will only grow. Assisted reproductive technologies can play a vital role in endangered species conservation. Many approaches are currently being utilized or explored, including animal cloning. Unfortunately, cloned animals are not genetically pure and therefore are not valuable for use in breeding programs.

Optimization of wood bison interspecific somatic cell nuclear transfer and embryo culture for species conservation

Interspecies somatic cell nuclear transfer (iSCNT) is a promising technique whereby a bison donor cell is transplanted into a domestic cattle oocyte. Different fibroblast donor cells have been demonstrated to have different embryonic outcomes. Problems with SCNT embryos have been also reported after in vitro culture. Toronto Zoo’s efforts have focused on wood bison iSCNT, a threatened Canadian species, in order to conserve its germplasm.

Influence of nuclear cytoplasmic interactions on developmental competence of bison

Somatic cell nuclear transfer (SCNT) is a unique technology that allows the preservation of the entire genome from an individual, thereby avoiding dilution of valuable alleles. This is an important criterion in endangered species preservation. Our interest in the Canadian wood bison, a threatened species, has brought us to consider the application of SCNT as a method for embryo production and genome preservation. This project will evaluate the developmental competence of bison embryos produced by interspecies SCNT, whereby a bison donor cell is transplanted into a domestic cattle oocyte.

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