Advanced differential mobility mass spectrometry with electron capture dissociation for the characterization of therapeutic proteins - Year two

Many new pharmaceuticals are based on large biomolecules like proteins. Even small differences in the protein structure can cause significant changes in the efficacy and safety of these drugs. Furthermore, these large molecules are difficult to characterize without advanced instrumentation and methods. Current technologies still struggle with robustness and reproducibility. This study aims to introduce new technology to improve the reliability of protein pharmaceutical characterization. It will leverage a separation technique called differential mobility spectrometry and utilize a gas-phase chemical modification called hydrogen deuterium exchange to characterize and verify the structure and shape of various proteins. These technologies will increase our ability to identify novel modifications and structures much more quickly while simultaneously reducing our reliance on less robust liquid-based separation methods. Developing this application will provide the additional push needed to further commercialize and market differential mobility spectrometry equipment.

Intern: 
Brendon Seale
Faculty Supervisor: 
Derek Wilson
Province: 
Ontario
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