Bacterial cellulose (BC) is a natural polymer produced by certain bacteria in the form of nanofiber. Being a natural nanobiomaterial, it has been investigated for a broad range of applications ranging from headphone diaphragm to wound dressing and medical implant. The biochemical process for BC production using the well-established bacterium, Gluconacetobacter xylinus, is limited by the kinetics and oxygen availability resulting in a modest yield and long production time. In this project, we will investigate a recently discovered facultative bacterium for BC production. This bacterium has the potential of increasing the efficiency of BC production by more than ten folds thus significantly increase the cost effectiveness of virtually all BC applications. The BC production process will be optimized for maximum yield and minimum production time. Product BC nanofibers will be characterized. Wound healing applications in the form of a regular and an antimicrobial wound dressing will be developed.