Baseline parameters for application of human 3-D organoids as an in vitro model for studying neurotropic viral infection
Viruses that infect the brain, such as Zika virus, are a significant threat to public health in Canada since many are poorly understood, and no vaccines or antivirals exist to combat them. We are investigating the use of “mini-brain” organoids derived from human stem cells as a model to study Zika virus infection that mimics the physiology of the human brain. Here, we seek to optimize our methodology for infecting brain organoids with Zika virus. This work will form an important foundation for future research into Zika virus and other brain-targeting viruses, and will facilitate development of vaccines and antivirals to protect Canadians.