Bioorthogonal spin labeling approaches for high sensitivity electron paramagnetic resonance spectroscopy
In order to investigate proteins in their natural environment one can attach tiny reporter molecules to them that can be traced with appropriate instruments. However, these small reporter molecules may often cause strong perturbations to the functionality of the proteins, or cannot be seen due to experimental restrictions like low concentrations. Bioorthogonal chemistry aims to eliminate such experimental restrictions by using as inert molecules as possible to see how proteins really work. We want to test novel bioorthogonal strategies for attaching reporter molecules that can be detected with electron paramagnetic resonance (EPR) spectroscopy, a powerful magnetic resonance method that is able to detect changes in structure and function of proteins. As we also have a unique and highly sensitive, state-of-the-art EPR device at hand, together with new bioorthogonal strategies we are convinced that we will bridge the gap to a new world of applications.