Crif1 : Protein expression, purification and crystallisation
Human Crif1 is a protein with multiple functions, playing important roles in embryonic development, cellular stress, cell cycle regulation and mitochondrial membrane integrity. CRIF1 is coined to play a regulatory role in the Bone Marrow microenvironment-induced leukemia cell cycle arrest possibly through interacting with CDK2 and acting as a cyclin-dependent kinase inhibitor.
In this project, we will express human Crif1 protein in baculovirus system, purify it with affinity chromatography and followed by crystallisation. The structural elucidation of Crif1 will enhance the understanding of complex processes in leukemia cell cycle arrest and improve the strategy for drug research.