Interrogation of small RNAs in MSC chondrogenic potency
Injuries involving joint cartilage such as osteoarthritis (OA) are some of the most common causes of lameness and pain in equine. Sophisticated means of monitoring joint health status are needed to allow early detection and intervention as well as monitoring the effect of interventions. MicroRNAs (miRNAs), a class of short non-coding RNAs, participate in various biological processes including cartilage development and homeostasis. miRNAs have been measured at the tissue level, in synovial fluid and serum, and may reflect some aspects of the health status of the animal. In this research we aim to identify novel miRNAs which can be used as “non-invasive” markers to monitor chondrogenic potency as well as cytogenetic stability of stromal cells in equine.
This research may identify unique biomarkers of joint health that could be evaluated through frequent blood and/or joint fluid sampling and analysis. The results of this project in long-term, may pave the way in providing new and innovative treatment options for patients with cartilage deficiencies.