METAGENOMIC SEARCH FOR GLYCOSIDEPHOSPHORYLASES USING A PHOSPHATE DEPENDENT2,4-DINITROPHENYL GLYCOSIDE COLORIMETRICASSAY

Carbohydrate active enzymes are used widely in the commercial sector and in industries ranging from food processing to biofuel generation. Of these enzymes, the class known as glycoside phosphorylases has seemingly been overlooked by industry even given their noted advantages over related classes currently in use. Given the fact that a limited number of these enzymes have been identified, we think this presents a timely niche opportunity to develop a commercially available suite of these enzymes in collaboration with MetaMixis. In previous work we have developed a functionalscreening methodology that allows use to probe the metagenomic libraries available through MetaMixis for glycoside phosphorylase activity. This proposed research outlined above aims to broaden our functional screening capabilities to identify additional glycoside phosphorylases that act on a wide range of substrates. It is our hope to develop a resource that can be applied to a wide range of industrial purposes.

Intern: 
Spence Macdonald
Faculty Supervisor: 
Dr. Stephen G. Withers
Project Year: 
2014
Province: 
British Columbia
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