Mild traumatic brain injury (mTBI) and sports-related concussion have complex and variable neuronal pathophysiology. Despite extensive attempts for discovery and identification of protein biomarkers in the blood of concussion patients, the success of proteins markers is limited due to the heterogeneity and complexity of concussion. There are still no reliable protein biomarkers that can accurately diagnose sport-related concussion and to distinguish complicated and uncomplicated concussion. This project aims to perform quantitative mass spectrometry-based protein profiling for the discovery of diagnostic protein biomarkers of complicated and uncomplicated concussion patients. The project will represent a significant advancement in the health and quality of the life of patients diagnosed with concussion. The outcome of this project including the discovery biomarkers and protocols of testing biosamples will benefit the partner by providing IPs to explore partnerships with molecular diagnostic players for developing point-of-care devices for rapid detection of one or multiple mTBI protein biomarkers.