Simultaneous Analysis of Endocannabinoids with Mass Spectrometry Methods in Human Serum using Point-of-care Collection Devices

Endocannabinoids are compounds produced by the human body that bind to and activate cannabinoid receptors. There are two major endocannabinoids: anandamide (AEA) and 2-arachidonoylglycerol (2-AG). Both play a role in regulating the firing of brain cells in the nervous system and attenuating immune system responses. As such, the endocannabinoid system is implicated in several clinically relevant processes including appetite regulation, pain management, fertility, and beyond. Routine measurements of AEA and 2-AG can enable further investigation by healthcare professionals into the impact supplementing the endocannabinoid system can have for patients. Method development for AEA and 2-AG will be explored using serum samples analyzed with liquid chromatography-mass spectrometry (LC-MS). A dried serum spot collection card will be evaluated for use as a point-of-care tool for healthcare professionals.

Jordan Holzschuher
Faculty Supervisor: 
Philip Britz-McKibbin
Partner University: