Standardization and optimization of saliva sample processing for SARS-CoV-2 detection without nucleic acid purification
The SARS-CoV-2 outbreak, which started in Dec. 2019, has so far not been contained due to unpreparedness and unsuccessful development of antiviral drugs against SARS-CoV-2. In response to this pandemic, we propose development of a diagnostic assay based on saliva samples. We will also standardize virus collection procedure and inactivation steps to reduce the turnaround time of the results. We have the required expertise of working with virology techniques, molecular biology and diagnostic assay development. The goal of our design will be to 1) standardize sample collection buffer; 2) standardize additive which is most effective for inactivation of SARS-CoV2; 3) Standardize isolation buffer to skip the RNA extraction step; 4) Reduce the turn-around time for result.