Development of sc-RNA based methods for mAb identification and validation - Year two

The overall goal of this project is to develop more cost-effective methods for generating monoclonal antibodies (mAb) against cancer biomarkers. While the traditional protocols for mAb generation used by MédiMabs have been established decades ago, recent technological developments have opened the door to new, and potentially far more efficient, methodologies. In this project, we will research the use of single cell DNA sequencing as a method to rapidly identify mAb producing cells without performing traditional hybridoma fusions.

Development of sc-RNA based methods for mAb identification and validation

The overall goal of this project is to develop more cost-effective methods for generating monoclonal antibodies (mAb) against cancer biomarkers. While the traditional protocols for mAb generation used by MédiMabs have been established decades ago, recent technological developments have opened the door to new, and potentially far more efficient, methodologies. In this project, we will research the use of single cell DNA sequencing as a method to rapidly identify mAb producing cells without performing traditional hybridoma fusions.

Encapsulation efficiency and viability of phage-coated implants for the prevention and treatment of periprosthetic joint infections in high risk patients

The widespread use of antimicrobial compounds in recent years has led to a pronounced increase in antimicrobial resistance (AMR) in bacteria. These resistant organisms require increased doses of strong antibiotics for prolonged periods for effective treatment. This is such a significant problem that the world health organization has put in place a global action plan to combat this phenomenon.

Implication de nouveaux produits biologiques sur la réponse du stress au réticulum endoplasmique chez les cellules myéloïdes humaines - Year Two

Ce projet vise à élucider davantage le mode d’action de certains produits élaborés par ProMetic BioSciences inc. sur certaines cellules myéloïdes humaines en relation avec une réponse cellulaire impliquée dans un nombre grandissant de maladies humaines. Il s’agit d’un stress du réticulum endoplasmique (RE), un organite cellulaire impliqué dans la synthèse des protéines. Cette dernière est à la base même de la vie non seulement d’une cellule, mais également d’organismes pluricellulaires comme l’homme.

Ingénierie de la lipase B de Candida antarctica pour l'estérification d'acides aromatiques encombrants

Les esters à chaînes courtes sont des molécules employées comme ingrédients de base dans la fabrication d'arômes et de fragrances. Certains esters sont très prisés, tels que ceux entrant dans la composition des arômes de menthe givrée et de fraise. Cependant, ces esters sont particulièrement difficiles à obtenir naturellement et/ou artificiellement. De plus, la demande commerciale est grande pour obtenir ces esters avec une certification naturelle. Il est néanmoins possible de les fabriquer ''naturellement'' en utilisant des enzymes en laboratoire.

Prevention of hepatitis C virus (HCV) re-infection after liver transplantation

Over 170 million persons worldwide are chronically infected by hepatitis C virus (HCV), leading to endstage liver disease and cancer. HCV is the single most common indication for liver transplantation in Canada and the rest of the Western world. After transplant, HCV re-infection is universal and is the chief cause of graft loss. Treatment of post-transplant recurrent HCV is more difficult than pretransplant, with lower response rates that partly reflect the effects of immunosuppression.We propose that the ideal management of post-transplant HCV is its prevention.

Identification of bacterial species with antimicrobial activities against Xanthomonas perforans and optimization of their metabolite production.

The most active bacterial species showed the highest antimicrobial activity against Xanthomonas perforans XcT4 will be identified and tested for their metabolite activities using plant bioassay. Two methods of tomato seedlings treatment will be used: soaking of tomato seeds in bacterial cell suspension before sowing in pots with soil; and foliar spraying of seedlings by bacterial metabolites during their growth. Contamination of seedlings by X. perforans XcT4 will be done after metabolite treatment.

Development of therapeutic peptides to treat bacterial and viral infections Year Two

Advanced Theranostics develops therapeutic peptides to treat bacterial and viral infections. Synthetic peptides have two major disadvantages; ie. they are expensive to synthesize and they are broken down quickly in the body. The solution is to attach these peptides to a human scaffold protein such as albumin, which is abundant in human serum, and to make these peptides as fusion proteins using recombinant DNA technology.

"The mechanism(s) of antimicrobial activity of Kisameet clay

Kisameet Clay is a First Nations product of great interest due to its potent antimicrobial activity. Investigation of KC, as a potent inhibitor of bacterial and yeast growth, shows promise as a therapeutic agent. Work is in progress to identify the chemical components of KC responsible for this therapeutic activity. Work is in progress to identify the active substances. We are collaborating with experts in the study of clay-like materials to ascertain the role of metal ion interactions in the antibacterial property.

Effets de combinaisons des fusariotoxines: la zéaralénone (ZEN), le déoxynivalénol (DON) et la fumonisine B1 (FB1) sur le fonctionnement cellulaire et les voies de signalisation : Étude préventive

Les conditions climatiques et géographiques et les particularités socio-économiques de la population ont fait du Canada un pays propice au développement d’une flore fongique sécrétrice de toxines naturelles, les mycotoxines. Ces mycotoxines s’avèrent de puissants contaminants de l’environnement et particulièrement de la chaîne alimentaire. A l’heure actuelle, la situation est davantage aggravée par la multi-contamination des denrées qui complique les effets toxiques par des effets additionnels ou synergiques.