Development of a wireless mini-microscope for the study of brain function - Year two

In the last years imaging using miniature microscopes (mini-microscopes also called mini-endoscopes) has become a method of choice for the understanding of activity at the cellular and network levels in different brain regions, in freely behaving animals. However, mini-microscopes are connected with wires to other devices, which largely alleviate the use of these imaging systems for the long-term imaging and monitoring of animals’ behavior during complex behavioral tasks.

Characterization of the lateral hypothalamic neural outputs using camera-based multi-fiber photometry, part 2

Understanding how brain processes information is the one of the most intriguing questions that modern neuroscience faces. This project is aimed to characterize how the lateral hypothalamus process information to downstream brain targets to guide proper behavioral responses. This brain area is well-known to regulate key aspects of the behavior. But how does it process information to control behavior? To answer this question, we use and develop a state-of-the-art technique called fiber photometry.

Characterization of the lateral hypothalamic neural outputs using camera-based multi-fiber photometry

Understanding how brain processes information is the one of the most intriguing questions that modern neuroscience faces. This project is aimed to characterize how the lateral hypothalamus process information to downstream brain targets to guide proper behavioral responses. This brain area is well-known to regulate key aspects of the behavior. But how does it process information to control behavior? To answer this question, we use and develop a state-of-the-art technique called fiber photometry.

Development of a wireless mini-microscope for the study of brain function

In the last years imaging using miniature microscopes (mini-microscopes also called mini-endoscopes) has become a method of choice for the understanding of activity at the cellular and network levels in different brain regions, in freely behaving animals. However, mini-microscopes are connected with wires to other devices, which largely alleviate the use of these imaging systems for the long-term imaging and monitoring of animals’ behavior during complex behavioral tasks.

Mise au point d’un microscope miniature double pour imagerie bihémisphérique de cerveaux de souris.

La compréhension du système nerveux, plus particulièrement du cerveau, suscite beaucoup d’intérêt depuis toujours. Décoder l’organisation cellulaire ainsi que les modes de communication neuronaux demeurent par contre un énorme défi. La microscopie optique jumelée au développement de sonde fluorescente a permis de faire d’énorme progrès en neuroscience. Avec la venue de l’optogénétique et la capacité d’enregistrer ou de contrôler les cellules nerveuses, il est maintenant possible in vivo d’observer le comportement de rongeurs tout en agissant sur des populations neuronales bien précises.

Développement d’un microscope miniature pour l’étude de l’activité neuronale dans des animaux se déplaçant librement

Un but fondamental des neurosciences est la compréhension des opérations de base du cerveau, et comment celles-ci sont affectées par les changements comportementaux. Plus précisément, de quelles façons l’activité neuronale est-elle liée aux processus cognitifs et comportementaux ? Depuis son développement, la microscopie optique a toujours été un instrument de choix pour étudier les propriétés cellulaires du cerveau. Couplée aux nombreuses sondes fluorescentes disponibles, elle permet d’élucider les questions relatives aux neurosciences, tant au niveau structurel que fonctionnel.