Assessment and selection of competent oocytes for embryo development
Research project aims:
1) To capture and compare microRNA (MIR) signatures in mouse ovarian follicular fluid and spent media of cumulous cells-oocyte complexes (COCs) isolated from infertility mouse models to identify one or more unique MIR signatures that will distinguish competent oocytes from incompetent oocytes.
2) Preliminary design and validation of an oocyte developmental competence diagnostic using quantitative PCR (qPCR) arrays. The scope of this aim will also include development of the software protocol required to perform the qPCR array analyses. Identify and recommend other possible point-of-care (POC) hardware technologies that may be incorporated to improve the POC diagnostic tool ease-of-use.
3) Establish the protocol procedures required for collection of both human COC spent media and human ovarian follicular fluid and define the clinical data collection and storage architecture requirements for a future clinical human evaluation of the oocyte developmental competency diagnostic tool at the Montreal Reproductive Clinic (MRC).