Assessing the Efficiency of Extracellular Vesicles for the Therapeutic Delivery of Modified mRNA

Extracellular vesicles (EVs) are tiny, membrane-based particles that cells release into various bodily fluids. They serve as messengers, facilitating communication between different cells in the body and, therefore, playing pivotal roles in modulating various biological processes. They achieve this by transporting a diversity of bioactive cargos, including RNA molecules. Over the past decade, our understanding of EVs has rapidly expanded, encompassing their different classes and unique attributes, as well as their roles in both normal physiological functions and pathological conditions. In the context of mRNA-based pharmaceuticals, driven by the success of Covid-19 vaccines, there is a growing need for innovative mRNA therapies across numerous diseases. While lipid nanoparticle (LNP) formulations are commonly used for drug delivery and have demonstrated effectiveness, our project will explore alternative RNA delivery methods that can complement and enhance current approaches. To address this, we are harnessing EVs as natural mRNA carriers, capitalizing on their established safety and specificity. The primary objective is to develop an efficient method for loading mRNA into EVs, transforming them into precision delivery vehicles to enhance therapy efficacy. Project milestones include demonstrating efficient mRNA loading, developing methods to produce, characterize, and compare EVs to LNPs, and assessing safety and efficacy in pre-clinical models. Upon completion, this project will produce essential data to back future development efforts, offering patients alternative and more effective mRNA therapies. Simultaneously, it will grant industry partners access to cutting-edge delivery technology, solidifying Québec’s status as a leading hub in biopharmaceutical innovation.

Faculty Supervisor:

Eric Lécuyer;Jean-François Côté

Student:

Partner:

RNA Technologies & Therapeutics Inc.

Discipline:

Life Sciences

Sector:

Professional, scientific and technical services

University:

Université de Montréal

Program: