Cloning, expression and characterization of a novel gene involved in Amaryllidaceae alkaloid metabolism

Amaryllidaceae alkaloids (AAs) are a group of specialized metabolites found predominantly in the Amaryllidaceae plant family. Approximately 650 naturally occurring AAs have been identified, many of which possess a variety of potent biological and pharmacological properties. In contrast to the large body of knowledge on the pharmacological and phytochemical aspects of AAs, their molecular and physiological features are less explored. A better understanding of AA biosynthesis and metabolic regulation is crucial to take advantage of new metabolic engineering technologies for improving the efficiency and sustainability of plant or microbial AA production.This internship will focus on the characterization of tyrosine decarboxylases (TYDC) potentially involved in the biosynthesis of AA. Two TYDC transcripts were identified in the transcriptome of Amaryllidaceae plants but their function remain unknown. This project will help to understand AA biosynthesis to provide tools for alternative biotechnologies for the production of high-valued AAs.

Faculty Supervisor:

Isabel Desgagné-Penix

Student:

Partner:

Universidade Federal do Espírito Santo

Discipline:

Life Sciences

Sector:

Biotechnology; Life Sciences (not health); Pharmaceuticals

University:

Université du Québec à Trois-Rivières

Program: