Hybrid RNA sequencing of Douglas-firs exposed to simulations of future extreme climate events

Douglas-fir is one of the most economically important tree species in Canada and it is at risk of maladaptation to local environment due to climate change. Thus, the aim of my project is to understand the adaptation of Douglas-fir to future extreme climatic events. Our group at Ensminger Lab at University of Toronto monitored the performance and survival of four different Douglas-fir genotypes under simulations of future drought and heat events. During the experiment, we also collected samples for long- and short-read gene sequencing. In collaboration with Jill Wegrzyn, one of the world’s leading conifer genome researcher, we will create novel hybrid gene sequencing assemblies. We will also develop new algorithms to identify genes that confer improved performance in Douglas-fir under extreme climatic stress. This work will improve our understanding of plant responses to climate stressors and provide genomic tools for the identification of Douglas-fir genotypes that are adapted to perform better in a future climate. Ultimately, these tools can be used to inform tree improvement programs, and forest management practices and conservations.

Faculty Supervisor:

Ingo Ensminger

Student:

Partner:

University of Connecticut

Discipline:

Life Sciences

Sector:

Education

University:

University of Toronto

Program: