Implication of Epigenetic Dysregulation in Early Embryo Development

The project aims to identify the interdependence that exist between epigenetic modifications that regulate gene expression, as well as their susceptibilities to permanent disruption following errors in the embryonic program. We use an innovative embryonic stem cell model in which the expression of an epigenetic enzyme responsible for DNA methylation can be controlled. We will define at high resolution the chronology and evolution of epigenetic disruption in embryonic cells. Beyond standard techniques molecular biology, sequencing approaches (Methyl-Seq, ChIP-Seq) coupled with bioinformatic analyzes will be exploited to establish detailed signatures for the DNA methylation and histone modifications. Under the guidance of a senior Postdoc, the student will participate in various aspects (experiments, analyses) of this project. By finely dissecting the epigenetic dysregulation mechanisms in embryonic stem cells, we aim to identify which epigenetic modifications are likely to be dysregulated in early embryos and how this could lead to adverse development.

Faculty Supervisor:

Serge McGraw

Student:

Partner:

Universidad Nacional Autónoma de México

Discipline:

Life Sciences

Sector:

Education

University:

Université de Montréal

Program: