Uploading of intercellular tension induces the directional translocation of PKC?

Endothelial cells( ECs) form the inner surface of blood vessels. If they break down, they may lead to vascular disease, heart failure,and high blood pressure. Endothelial cells(ECs) functions are regulated by permanent mechanical stresses such as shear stress and pressure from blood stream. When some cells are damaged, the adjacent cells are induced by them, and they react to this change. Protein Kinas C? (PKC?), which is widely expressed in mammalian tissues, plays a crucial role in regulation of cells, when the cells are damaged. PKC? has a critical role in disease treatment such as cardiovascular dysfunctions, arterial thrombosis, cancer, and would healing. During wound healing process, this protein (PKC?) translocates to the membrane through Ca+2 waves. Ca2+ waves propagate within a single cell are called intracellular Ca2+ waves, and coordinate multicellular responses.
In this original project, we will perform mechanical wounding on a single cell in oder to disrupt intercellular adhesion and observe the activity of PKC? in the adjacent cell. This wounding will be done by puncturing the cell with a micropipette.

Faculty Supervisor:

Lyes Kadem

Student:

Partner:

Kyushu University

Discipline:

Engineering

Sector:

Education

University:

Concordia University

Program: