Developing a protocol for single cell Hi-C sequencing in human post-mortem brain

The purpose of the proposed research travel is to co-develop a working protocol for high-throughput chromatin conformation capture (Hi-C) sequencing using human post-mortem brain tissue at the single-nucleus level. I will be traveling to the lab of Schahram Akbarian (MD, PhD) at the Icahn School of Medicine at Mount Sinai in New York City. Dr. Akbarian is an international leader in studying the epigenetics of the human brain. Furthermore, Dr. Akbarian has immense expertise in studying the genomic organization of specific cell types in the brain. The development of this protocol has the potential to change the way we study the brain and contribute largely to how we define and categorize different cell types. Furthermore, we will be able to interrogate how genomic organization changes in a disease state, and how this change effects gene transcription and other downstream mechanisms. I will also have to opportunity to bring this protocol back to my lab, at McGill University, were I will train others in implementing this protocol for their own projects.

Faculty Supervisor:

Gustavo Turecki

Student:

Partner:

Icahn School of Medicine at Mount Sinai

Discipline:

Life Sciences

Sector:

Life Sciences (not health); Health and Related Sciences & Technology; Education

University:

McGill University

Program: