Microfluidic phage display platform for ligand evolution in 3D cellular constructs and engineered phage therapeutics delivery

This project aims to develop a microfluidic device that can identify cancer-targeting molecules capable of penetrating and retaining tumor tissue. Traditional laboratory methods identify the targeting molecule using 2D cell cultures or solely protein as the target model. However, neither of these models can accurately represent the structure and conditions of real tumor tissue, leading to the failure of most candidates in clinical trials. To minimize reagent use and make the device low-cost and portable, I use consumer-level 3D printing to fabricate the mold for the microfluidic chip. 3D cancer cell models that reproduce tumor physiology were trapped in the chip to screen for molecules that bind and penetrate the models under realistic conditions. The mature device and screening workflow will be transferred to the host institution, where advanced biological validation can be conducted using complementary expertise and facilities. This collaboration allows the Canadian institution to advance microfluidic engineering and screening technology development, while enabling the host institution to apply the platform to downstream validation and broader experimental applications. Together, the project establishes a transferable, reusable screening framework that supports international collaboration and enhances cancer-targeting research.

Faculty Supervisor:

Maryam Tabrizian

Student:

Partner:

The Chinese University of Hong Kong

Discipline:

Engineering

Sector:

Education

University:

McGill University

Program: