Studying formation and destabilization mechanisms of pickering emulsions encapsulated with bioactive compounds via confocal/STED microscopy

Bioactive compounds are naturally occurring food components that can promote human health, but they often lose their effectiveness because of problems such as low stability, low solubility, and poor absorption in the body. Encapsulation in emulsions is one promising way to protect these compounds and help them reach their target tissues, but the main limitation is that emulsions are not always stable and can separate over time. Pickering emulsions, which are stabilized by solid particles rather than traditional surfactants, have been shown to offer better stability and lower toxicity, making them highly attractive for food and nutraceutical applications. However, there is limited understanding of the exact mechanisms by which they become unstable, particularly when bioactive compounds are encapsulated inside them. To address this knowledge gap, the project will use advanced imaging methods, such as confocal and STED microscopy, to capture high-resolution images of emulsions at different stages of formation and destabilization. The images will be analyzed using quantitative tools to provide information on droplet size, shape, distribution, and the arrangement of encapsulated compounds. By combining this information, the project will build new understanding of how emulsion stability is related to its microstructure and the role of protein nanoparticles as stabilizers.

Faculty Supervisor:

Nandika Bandara

Student:

Partner:

University of Copenhagen

Discipline:

Life Sciences

Sector:

Education

University:

University of Manitoba

Program: