Studying the structure of four-stranded DNA

Our DNA exists in many different forms. The G-quadruplex is a type of DNA structure made from four strands of DNA. These structures are highly stable and potentially form throughout the genome at clinically important sites. To understand how G-quadruplex structures form, we need to investigate the physical forces that hold the molecules together. When we monitor the change in G-quadruplex structures exposed to high temperature and high pressure, we are better able to understand the energetics of the folding and unfolding transition. Many of the tools used to study this DNA in depth are not available in Canada, especially the tools that enable high-pressure biophysical studies. With the equipment available in Japan, we will be able to better understand the change in volume of solutions containing G-quadruplexes. This opportunity will enable us to produce publishable data, thereby enhancing our understanding of non-canonical DNA structures.

Faculty Supervisor:

Robert Macgregor

Student:

Partner:

Ritsumeikan University

Discipline:

Physics

Sector:

Biotechnology; Pharmaceuticals

University:

University of Toronto

Program: