Development of models to monitor vitamin D activity
Vitamin D compounds are being developed for the treatment of chronic kidney disease. The purpose of this proposal is to develop a cell-based screening platform that will allow the rapid assessment of relative efficacy of a library of compounds. The intern will use recombinant DNA methodology to generate a cell-based assay in which the green fluorescence protein will be inserted into a vitamin D responsive gene; thus, permitting the visualization of vitamin D signaling in real-time. This same recombinant DNA construct will be designed in such a way that it can be further used to establish a line of mice in which tissues responding to vitamin D compounds will “glow”. This may permit the visualization of pharmacodynamic properties of compounds in live animals.
Voir la description complète du projetGlenville Jones
Cytochroma Inc
Life Sciences
Professional, scientific and technical services
Queen's University
Accelerate