Complex Microscopy

The proposed project is to combine digital holographic microscopy (DHM) and interferometric scattering microscopy (iSCAT), which can be separated into two sub-tasks. The first one is introducing reconstruction holographic algorithm to iSCAT image analysis, realizing real-time particle 3D tracking. Tracking objects along the optical axis in iSCAT is commonly done by comparing the shape of its interferogram with a pre-recorded calibration table. With the adoption of field reconstruction algorithms, the same result can be obtained with a calibration-free procedure, without the need for pre-knowledge of the sample. This is especially relevant in the biological context, where evolving samples are observed over longer periods of time. The second task is to add off-axis DHM channel to the existing iSCAT-TIRF setup. The extra channel will allow for more informative measurements to be performed simultaneously, which is possible to obtain the complex properties of the sample, i.e., refractive index.

Faculty Supervisor:

Christopher Yip

Student:

Partner:

Friedrich-Schiller-Universität Jena

Discipline:

Engineering

Sector:

Education

University:

University of Toronto

Program: