Cryosectionable Well Inserts for Histology of 3D Cultures

In this research project a device is being developed and tested for its ability to permit the analysis of growing human tissues. Recently scientist have started to believe that cells need to be grown in 3D just like they are in our bodies. Similar to a cup, this device can be used to contain growing human tissues. The novel aspect of this cup is that it is made of a material softer than plastic that can be removed from standard culture plates and be cut with standard tissue analysis cutting devices. Over a period of 15 days, cells will be grown in the biomaterial (held in the cup) and will be removed and frozen on days 0,5,10, 15. Cutting the frozen cups containing the biomaterial and cells, the scientist can make thinner than paper slices that can be analyzed with various microscopy techniques. TO BE CONT’D

Faculty Supervisor:

Simon Tran

Student:

Partner:

University of Helsinki

Discipline:

Life Sciences

Sector:

Education

University:

McGill University

Program: