Derivation of germ lineage cells from bovine haploid parthenogenetic embryos

Artificial reproductive technics (ART) have been widely utilized to maximize the use of genomics for animal improvement. In the forefront of novel expectations in ART is the ability to derive sperm and oocytes in vitro by differentiating pluripotent stem cells into germ cells and then into gametes. However, a major hurdle in the derivation of gametes from diploid pluripotent stem cells is to achieve complete meiosis and derive haploid cells that become viable sperm and/or oocytes. Our main goal in this proposal is to derive haploid primordial germ cells from parthenogenetically-derived embryonic cells that can differentiate into haploid primordial germ cells with epigenetic markings suited to produce genomically characterized diploid embryos. Apart from creating an opportunity to develop novel knowledge on the role of haploid genomes in mammalian development, this project will provide a unique tool to accelerate genetic gain by improving the selection accuracy of cattle breeding programs. The proposed project will be performed with close interaction with research staff from SEMEX, thereby enabling that all novel technical and scientific knowledge be effectively transferred to the industry

Faculty Supervisor:

Lawrence Smith

Student:

Partner:

L'Alliance Boviteq Inc

Discipline:

Life Sciences

Sector:

Agriculture; Professional, scientific and technical services

University:

Université de Montréal

Program: