Developing chromosomal integration methods for functional screening and metabolic engineering

The goal of this project is to further develop the JGI technology CRAGE, which enables the insertion of large DNA fragments into the chromosome of a host organism. Over the course of the 3 months, the CRAGE system will be adapted for the E. coli EPI300 strain that is host to the Hallam Lab functional screening platform. By establishing the CRAGE system in EPI300, this project will allow us to increase the sensitivity and efficacy of functional screening, allowing us to more effectively discover enzyme with novel biocatalytic functions. The advancements will also increase our capacity to engineer metabolic flux within these organisms and to optimize them for downstream industrial applications. TO BE CONT’D

Faculty Supervisor:

Steve Hallam

Student:

Partner:

University of California, Merced

Discipline:

Life Sciences

Sector:

Biotechnology; Environmental Science and Technology; Life Sciences (not health)

University:

The University of British Columbia

Program:

Globalink Research Award

Related projects

Discover more projects across a range of sectors and discipline — from AI to cleantech to social innovation.

View all
Current openings

Find the perfect opportunity to put your academic skills and knowledge into practice!

Find Projects

Mitacs Partners

The strong support from governments across Canada, international partners, universities, colleges, companies, and community organizations has enabled Mitacs to focus on the core idea that talent and partnerships power innovation — and innovation creates a better future.

Government
Academic
Industry
Innovation
International

International

Load More

International

Load More

Join a thriving innovation ecosystem.

Subscribe to our newsletter now!

Subscribe

Mitacs — Return on Innovation

Mitacs is funded by the Government of Canada, the Government of Alberta, the Government of British Columbia, Research Manitoba, the Government of New Brunswick, the Government of Newfoundland and Labrador, the Government of Nova Scotia, the Government of Ontario, Innovation PEI, the Government of Quebec, the Government of Saskatchewan, and the Government of Yukon.