Development of a Cell-Based Signaling Assay to Measure the Potency of a Therapeutic Antibody

Cytokines are proteins that can modulate cellular activity by binding to membrane-bound receptors or soluble receptors. When a cytokine binds to a soluble receptor, it follows the trans-signaling pathway. This signaling pathway induces unique effects on cellular activity which can drive disease progression. This proposal describes the development of a cell-based assay that can be used to test the potency of an inhibitor that prevents a cytokine from binding to its soluble receptor. A specific receptor will be introduced into a cell line to study the activity between the cytokine and the receptor. Then, the inhibitor produced by HyperMabs will be tested in this assay against appropriate controls. This will enable the company to quantify the potency of its candidate. Furthermore, the assay produced in this project will be useful for assessing the potency of future biologic candidates produced by HyperMabs.

Faculty Supervisor:

Reza Salavati

Student:

Partner:

HyperMabs Inc

Discipline:

Life Sciences

Sector:

Professional, scientific and technical services

University:

McGill University

Program: