Development of a molecular assay for determining the number and viability of vaccine organisms

This project is designed to evaluate/test the viability of commercial live coccidial poultry vaccine. The efforts will be made to enumerate individual species within a mixture of several to many vaccine species. The current research revolves around viability-qPCR that may be able to simultaneously amplify and quantify parasites in a variety of samples and concentrations. . The viability/qPCR to detect and quantify species complexes in a cost-effective manner would be useful for vaccine quality control. The ability to test samples for viability in vitro (in addition to quantifying the agents present) is of direct interest to vaccine manufacturers as well as diagnostic laboratories within Canada and globally

Faculty Supervisor:

Dr. John R Barta

Student:

Mian Abdul Hafeez

Partner:

CEVA

Discipline:

Biology

Sector:

Life sciences

University:

University of Guelph