Development of a novel, rapid tool for non-typhoidal Salmonella detection and risk assessment for poultry.

Salmonellosis is a significant concern to public heath. Salmonella contaminated food is the main source of human infections. Emergence of novel Salmonella serovars with increased pathogenic potential and multidrug resistance led the industry to look for better diagnostic tools for in-depth characterization of the organism. Moreover, rapid detection of Salmonella serovars prevalent in poultry industry is a timely necessity to prevent outbreaks and mitigate timely control and prevention measures. This research study proposes in-depth characterization of Salmonella isolates using Oxford Nanopore whole genome sequencing platform. Project data will provide rapid determination of serovars, antimicrobial resistance profile and virulent traits. A metagenomic approach will be developed and validated to detect the Salmonella serovars directly from diagnostic samples and eventually improve the overall turnaround time, as compared to the traditional culture method. The organization partner will be the main laboratory for the entire project. It will benefit by improving diagnostic workflows and strengthening the relationships with the poultry industry.

Faculty Supervisor:

Musangu Ngeleka

Student:

Partner:

Prairie Diagnostic Services

Discipline:

Life Sciences

Sector:

Agriculture

University:

University of Saskatchewan

Program: