Development of an integrated online enzymatic micro-fluidic chip for glycoprotein analysisusing Chip-Cub QToF mass spectrometry

Glycoprotein analysis is very important in different field such as cancer and pharmaceutical research,
as ‘more than 70% of the proteins are glycosylated. However, the sample preparation of glycoprotein
for glycan profiling is very tedious as includes many enzymatic steps that could go for -36 hrs. This
project aims to develop a one chip reactor that can be used to prepare the glycoprotein(s) for mass
spectrometry analysis. This reactor includes online multi-enzymatic steps to eventually have the
glycans that are expressed on the glycoprotein, characterized and relatively quantified using mass
spectrometry. This chip can only be used on Agilent chip-cub that combined with Agilent QToF mass
spectrometry, which will lead to develop their Immunoglobulin commercial kit to be general for any Nglycoproteins.

Faculty Supervisor:

James Dennis

Student:

Partner:

Agilent Technologies

Discipline:

Life Sciences

Sector:

University:

University of Toronto

Program:

Accelerate