Electrochemical detection of Micro-RNA

MicroRNAs (miRNA) can be used as biomarkers for different types of cancer diagnostics. Therefore, obtaining point-of-care (POC) device for miRNA detection is of high value. Conventional miRNA sensing strategy usually involves reverse transcription PCR or target recycling strategy and hence those techniques are not suitable for POC devices. In the proposed work, electrochemical sensing strategy will be used to detect miRNAs as electrochemical transducer can be easily fabricated for POC device. A gold electrode containing complementary capture probe is used for the miRNA detection. Using toehold mediated strand displacement strategy with MB-tagged DNA strand the miRNA is replaced. The concentration of MB will be directly co-related with target miRNA concentration.

Faculty Supervisor:

Leyla Soleymani

Student:

Partner:

Fraunhofer Institute for Cell Therapy and Immunology IZI

Discipline:

Engineering

Sector:

Nanotechnology; Biotechnology; Health and Related Sciences & Technology

University:

McMaster University

Program: