Evosep One LC optimization and absolute quantitation of proteins in human and mouse plasma using Multiple Reaction Monitoring and Parallel Reaction Monitoring-Mass Spectrometry

Proteins are sub-cellular molecules that provide important information of how well the body is functioning at a given time. Abnormal increase or decrease in the level of proteins can be indicative of the presence of a disease. Hence, developing tools to accurately measure protein levels in the human body is of utmost importance. Over the past few decades, a specialised instrument called the Mass Spectrometer (MS) has been used for this. The MS instrument can be used in a targeted and untargeted manner. In the untargeted mode, the instrument provides us information on what kind of protein is present in a sample, whereas in the targeted mode, scientists tell the instrument what it needs to look for. When used in the targeted mode, the MS can be used as a validation tool that re-confirms the presence of certain proteins in the sample. Additionally, it also provides information the exact amount of protein present
Although the targeted mode works very efficiently, it is time consuming and unreliable when the protein concentration is very low in the patient sample.

Faculty Supervisor:

Elias Georges

Student:

Partner:

MRM Proteomics Inc

Discipline:

Life Sciences

Sector:

Professional, scientific and technical services

University:

McGill University

Program: