Exploring the role of MRC2 in Multiple Sclerosis Pathology

This project investigates the role of Mrc2 in CNS (Central-nervous system) infiltration of pro-inflammatory immune cells using an EAE mouse model. Fresh and postmortem tissue will be provided by collaborators. Single cell suspensions from brain, spinal cord, spleen, and lymph nodes will undergo flow cytometry analysis, using different markers to identify distinct cell populations. Unsupervised clustering and dimensionality reduction techniques will be applied for data analysis. Immunohistochemistry will confirm the findings, examining demyelinating lesions and infiltrating cell types. The objective is to understand Mrc2’s involvement in early EAE stages and its impact on CNS infiltration by pro-inflammatory cells, potentially reducing demyelination. Expected outcomes include insights into Mrc2’s role and that Mrc2 deficiency leads to a decreased infiltration of pro-inflammatory cells in the CNS.

Faculty Supervisor:

Alexandre Prat

Student:

Partner:

University of Fribourg

Discipline:

Life Sciences

Sector:

Education

University:

Université de Montréal

Program: