Fabricate Hydrogel Scaffold Encapsulating Living Cells for Myocardial Infarction Repair

To solve the issue of complementing cell loss or stimulating angiogenesis for repairing MI, scaffolds encapsulating living cells, which aim at inducing cell proliferation and differentiation, are designed and then synthesized based on biocompatible and biodegradable materials. The experiments are performed by applying them to 3D cultured MDSCs for cell proliferation and differentiation study via immunofluorescence and RT-PCR etc. Also experiments of injecting scaffolds into MI rats for analyzing recovery of heart function are carried out. Methods such as immunofluorescence, Western Blot and RT-PCR etc. are to taken. Results are anticipated including relatively well cell viability and differentiation ability, and better heart function in MI animals after injecting scaffolds than those untreated. If anticipated results of both in vitro and in vivo study are obtained, the goal for improving cell viability and differentiation ability can be accomplished and effects on heart function moderation can be obtained after injected with scaffolds in rats for in vivo tests.

Faculty Supervisor:

Daniel Chen

Student:

Partner:

Harbin Medical University

Discipline:

Life Sciences

Sector:

Education

University:

University of Saskatchewan

Program: