Modelling Acute Respiratory Distress Syndrome (ARDS) in Precision Cut Lung Slices (continuation)

As researchers strive to reduce the number of animals used in their research, many are turning to precision cut
lung slices (PCLS), thin organ slices prepared from fully developed mouse lungs. They represent a “mini-model”
that closely resembles the original organ in both anatomical structure and rich cellular environment. Despite the
increasing interest, no commercial system exists to provide a easy introduction to this technique that currently
requires lots of resources and expertise to perfect. With this project, we strive to provide researcher with a turnkey
solution for PCLS experiments by standardizing the technique and providing both instrumentation, software and
all experimental protocols to perform PCLS experiments from start to finish. We will then use this new platform
to compare outcomes of LPS induced ARDS, a model of interest for COVID-19 research when studying whole
mice and PCLS.

Faculty Supervisor:

Ynuk Bossé

Student:

Partner:

SCIREQ Scientific Respiratory Equipment

Discipline:

Life Sciences

Sector:

Professional, scientific and technical services

University:

Université Laval

Program: