Neurologically relevant enzyme expression and engineering for novel enzymatic biosensor development

Enzymes are important biological catalysts which span all domains of life. They play vital roles in the metabolism and production of biomolecules in different organs. Our interest lies in using enzymes to develop biosensors to be employed in studying neurochemical processes occurring in the brain. The purpose of this project is express and characterize three different wild type enzymes and their mutants (Glycine oxidase, D-aspartate oxidase, D-amino acid oxidase). Enzyme expression and purification will be completed through standard biochemistry techniques while activity characterization will be assessed using spectrophotometric assays. Upon expression and characterization, these enzymes will then be used to develop enzymatic biosensors which will be employed for in vitro single cell and in-vivo animal model studies of neurochemical processes.

Faculty Supervisor:

Janine Mauzeroll

Student:

Partner:

University of Insubria

Discipline:

Life Sciences

Sector:

Biotechnology; Health and Related Sciences & Technology; Pharmaceuticals

University:

McGill University

Program: