Optimization of production and purification of Bacillin 20 from Bacillus thuringiensis MS20 to produce cannabis

Bacillus thuringiensis MS 20 produces a peptide, Bacillin 20, that has been shown to improve crop stress tolerance, enhance plant growth, stimulate the production of phytohormones and modify the plant root systems for better uptake of water and nutrients. The purification of Bacillin 20 from microbial broth culture is affected by the organic compounds present in the growth media and as such decreases the overall peptide yield when subjected to various purification steps. The proposed work aims to modify microbiological growth media by using minimal medium salts with other additives followed by centrifugation and lyophilization of the cell-free supernatant (CFS). The concentrated extract will be further fractionated by Flash chromatography and C18 cartridges and Bacillin 20 purified. This work will eliminate the organic solvent-based extraction step involved in Bacillin 20 purification and will enhance the overall recovery and yield of Bacillin 20.

Faculty Supervisor:

Donald Smith

Student:

Partner:

CXC

Discipline:

Life Sciences

Sector:

Agriculture; Professional, scientific and technical services

University:

McGill University

Program: