Selection and Characterization of DNA aptamers for macrolide ring closure reactions

A class of compounds the work will be done are tetrahydrofuran-incorporating polyketide macrolides. This class of compounds are natural products found in diverse marine and terrestrial environments, and it has been found to show potentially useful activity in a variety of biological assays. In order to explore and expand this family of macrolides, it is required to vary the chemical space around the core structure of these compounds. The synthetic pathway involves a ring closing olefin metathesis reaction.
The primary objective is to select for DNA aptamers that bound one or the other isomer of the macrolide shown in the proposal specifically. And, then to see if the ring closure experiment was carried out in the presence of the aptamer, (1) one isomer would be preferentially formed, and (2) the rate of formation of that isomer may be accelerated. TO BE CONT’D

Faculty Supervisor:

Dipankar Sen

Student:

Partner:

Indian Institute of Technology Madras

Discipline:

Life Sciences

Sector:

Biotechnology

University:

Simon Fraser University

Program: