Validation d’une méthode d’histologie virtuelle pour l’étude de l’unité neurovasculaire dans des modèles animaux de la maladie d’Alzheimer

The project aims to validate the effectiveness of “phase contrast” synchrotron (x-ray) imaging techniques in detecting very small-scale features (particularly amyloid plaques) commonly associated with the onset of Alzheimer’s disease. This will be done though comparison of synchrotron-generated images of Alzheimer-afflicted brain tissue, against their optical microscopy image counterparts. The experimental process will involve five stages:
1 – The acquisition of high-resolution synchrotron x-ray imagery of brain of mice that are genetically modified to exhibit Alzheimer traits. (to be done by other researchers)
2 – The preparation of chemically-treated thins slices of brain tissue from the same mice, for the purposes of microscopic analysis.
3 – The acquisition of 2-D fluorescence images of the brain thins slices by means of an optical microscope.
4 – Identification of relevant features on 2-D fluorescence microscopy images, using image analysis software. TO BE CONT’D

Faculty Supervisor:

Baptiste Lacoste

Student:

Partner:

Université Claude Bernard Lyon 1

Discipline:

Life Sciences

Sector:

Education

University:

University of Ottawa

Program: