Widefield Time Resolved Fluorescence with Multi-Tap Pixel

We would like to develop a wide-field fluorescence lifetime imaging(FLIM) system with a 4-Tap image sensor developed by the Imaging Devices Laboratory. During the duration of our stay we will develop the software and hardware solution to enable the collection of wide field FLIM images, and test the performance on a standard biology set, and a local biological sample such as a root structure. We wish to show that a multi-tap pixel enables the efficient collection of multi-exponential decays. From these multi exponential decays we can demonstrate that a multi-tap pixel outperforms other time resolved solutions in low signal environments and is compatible for multipoint confocal microscopy. Our lab group has developed a widefield multipoint confocal microscope that uses window scanners to enable uniform scanning of 32×32 confocal points. TO BE CONT’D

Faculty Supervisor:

Qiyin Fang

Student:

Partner:

Shizuoka University

Discipline:

Engineering

Sector:

Education

University:

McMaster University

Program: